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. 1999 Dec 7;96(25):14430–14435. doi: 10.1073/pnas.96.25.14430

Figure 4.

Figure 4

The relationship of the 15q11-q13 IC to the nuclear matrix depends on its parental origin. The halo nuclei analyzed were those in which both copies of the chromosome 15 α satellite repeat (15cen, yellow) are present within the nuclear matrix (visible on 4′,6-diamidino-2-phenylindole counterstaining, blue), and one of the two copies of the HPRT probe (HPRT, green) is confined to the nuclear matrix with the homologue associated, at least in part, with the surrounding DNA halo. The IC probe is shown as the red signal in these images. (a) In a normal female, the majority (16/20) of analyzed halo nuclei show one IC signal solely within the nuclear matrix, the other partially within the DNA halo. The remaining DNA haloes (4/20) showed both signals to be present within the matrix. (b) In a patient with PWS caused by a deletion of the paternal 15q11-q13 region, the sole signal is from the maternal chromosome, found only in the matrix in the majority (18/20) of halo nuclei.