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TLR4 genotyping. A 415 bp DNA fragment was amplified by PCR using mouse tail DNA and two TLR4-specific primers, TLR4sen193 and TLR4ant607. The mutation in the TLR4 gene in C3H/HeJ mice creates an Nla III site in the 415 bp DNA fragment. Nla III digestion of the amplified DNA fragment from a mouse with the mutation produces a 304 and 111 bp DNA fragments. The restriction enzyme polymorphism is shown in the picture for each TLR4 genotype.
