Table 2.
Increased snf+ dose affects neither sex determination nor dosage compensation activities of a Sxl cDNA expression construct
P(snf+) dose | Phenotype of Sxl−/Y; P(hsp70:SxlcF1) tra+/tra− partially feminized males*
|
|||||||
---|---|---|---|---|---|---|---|---|
Viability relative to XY siblings with no P(hsp70:Sxl), (n siblings) | Sexual phenotype, n = 25; ±SEM
|
|||||||
Foreleg, percent intersex (n male sexcomb teeth) | Terminalia
|
Abdominal bristle number, male = 0
|
Pigmentation of hemitergites 5 and 6, percent all female (percent intersex) | |||||
Anus, percent all female (percent intersex) | Genitalia, percent all female (percent intersex) | (percent relative to pseudofemale control†)
|
||||||
7th hemitergite | 6th hemisternite | 7th hemisternite | ||||||
0 (class A) | 30% | 100% | 0% | 0% | 10.6 ± 0.4 | 7.9 ± 0.1 | 3.0 ± 0.1 | 86% |
(90) | (1.6 ± 0.2) | (100%) | (100%) | (45%) | (92%) | (58%) | (14%) | |
3 (class B) | 36% | 100% | 12% | 8% | 9.6 ± 0.5 | 8.3 ± 0.2 | 3.1 ± 0.2 | 91% |
(105) | (1.5 ± 0.2) | (88%) | (92%) | (41%) | (96%) | (61%) | (9%) |
From the cross: y w cm Sxlf1ct6sn3/Binsinscy, y w sn B; TM3,Ser P{hsp70:SxlcF1 w+mC}19/+ ☿☿ × ♂♂ w/Y; P{snf+w+mC}P{snf+w+mC} tra1 P{snf+w+mC}/tra1. The reduced dose of tra+ moderated the otherwise overly strong feminizing effect of hsp70:SxlcF1.
† Pseudofemales were tra1/tra1 males (XY) feminized by a transgene constitutively expressing female-specific tra+ product.