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TNF-α produced in response to mAGP depend on TLR2. RAW-TT10 cells transiently transfected with a control plasmid, MyD88-dominant negative (MyD88-DN), wild-type TLR2, or dominant negative TLR2 (TLR2-P681H) were analyzed for induction of TNF-α after stimulation with mAGP. Histograms are formatted as described in Fig. 1. Similar results were obtained in the presence or absence of polymyxin B.
