Figure 2.

Multilineage engraftment in all mice transplanted with muscle cells. Peripheral blood was drawn 6 and 12 weeks after transplantation and stained with antibodies against the Ly-5.1 marker, B220 (B cells), Thy-1 (T cells), and Gr-1 and Mac-1 (granulocytes and macrophages, respectively). Stained blood samples were analyzed by flow cytometry, as described in Experimental Procedures. The percentages of positive cells in each lineage are reported. Mouse 1 (M1) was killed after the 6-week analysis for subsequent study (Fig. 3).