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Flow cytometric analysis of HSC from secondary recipients of muscle cells. Bone marrow prepared from the secondary transplant recipient mouse 5 (Fig. 3) was stained with Hoechst 33342, anti-Ly-5.1-biotin, and Str-PE. (A and B) Whole bone marrow. (C and D) HSC. Whole bone marrow contained 0.03% HSC (determined by Hoechst dye efflux; refs. 21 and 22) and contained 63% Ly-5.1+ cells (B). The HSC population (C) contained 65% Ly-5.1+ cells (D).
