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. 1999 Dec 7;96(25):14499–14504. doi: 10.1073/pnas.96.25.14499

Figure 2.

Figure 2

RANTES–heparin complexes inhibit chemotaxis in differentiated HL-60 cells. HL-60 clone 15 cells were differentiated (see Materials and Methods) by the addition of butyric acid and IL-5. On day 7 of treatment the cells were harvested and assayed for chemotaxis in response to RANTES (A), RANTES–heparin complexes (B), or heparin alone (C). For these experiments, RANTES-GAG complexes were formed as described in the text by mixing RANTES (3 μM) with a 10-fold excess (30 μM) of clinical grade, endotoxin-free heparin (Fujisawa Pharmaceutical, Osaka). Serial concentrations of the complexes were tested in parallel with matching concentrations of either RANTES or heparin alone. Each assay condition was performed in triplicate. Each data point represents the mean value of triplicate assays. SD values are shown with bars.