Figure 3.

Binding of ¹²⁵I-RANTES–heparin complexes to glycanase-treated PBMC. Normal human PBMC were activated, harvested on day 6 of culture, and treated with glycanase mixture as described in Materials and Methods. (A) Binding of serial concentrations of ¹²⁵I-RANTES complexes (0.03–10 nM of the chemokine component) to treated cells. (B) Competition binding of 0.3 nM ¹²⁵I-RANTES complexes vs. serial concentrations of unlabeled RANTES complexes (0.01–100 nM chemokine component). Each data point represents the mean value of triplicate assays. SD values are shown with bars.