FIG. 3.
Construction of an arabinose-regulated spoT gene in Y. pestis KIM6+. The transcription terminator (TT) araC PBAD-Shine-Dalgarno (SD) sequence was inserted upstream of the spoT gene in the chromosome of ΔrelA233 KIM6+(pKD46). The steps involved are analogous to those described in the legend to Fig. 2. The positions of oligonucleotide primers, such as spoT-U1, spoT-P1, spoT-V1, and Cm-V, are indicated. (A) Insertion of sacB-cat TT araC PBAD-SD spoT just after rpoZ. (B) Excision of the sacB-cat cassette.