TABLE 2.

Composition and diversity of pufM PCR and RT-PCR libraries from the Delaware estuary in December 2001 and August 2002^a

Library	Total no. of clones	No. of groups	% Coverageb	Chao I
December 2001 river	76	33	57	65 (43, 129)
August 2002 river	95	23	76	37 (26, 79)
August 2002 river RNA	96	21	79	31 (22, 74)
August 2002 bay	107	26	76	61 (36, 152)

^aGroups were defined at 3% amino acid sequence divergence. The Chao I index was calculated using the DOTUR software program. Numbers in parentheses are 95% confidence limits.

^bPercent coverage was calculated with the formula [1 − (n/N)] × 100, where n is the number of singleton clones and N is the total number of sequences.