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. 2008 May 9;74(13):3969–3976. doi: 10.1128/AEM.00019-08

FIG. 2.

FIG. 2.

Detection limits of the Gull-2 PCR assay. (A) Plasmid DNA (10-fold dilutions of plasmid DNA ranging from 6 to 6 × 10−7 ng) containing a targeted insert in reaction mixtures with no fecal DNA background from lane 1 to lane 8 (negative control in lane 9). (B) Tenfold dilutions of plasmid DNA (6 to 6 × 10−7ng) containing a targeted insert in reaction mixtures spiked with bird fecal DNA (10 ng/μl) made of equal amounts of chicken, turkey, and Canadian goose fecal DNA extracts from lane1 to lane 8 (only bird DNA in lane 9). (C) Tenfold dilutions of seagull fecal DNA ranging from 6 to 6 × 10−7 ng/PCR mixture from lane1 to lane 8 (negative control in lane 9).