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Optimization of φEF24C codon usage to its host codon usage and its possible tRNA function. (A) Comparison of codon usage between φEF24C and E. faecalis V583. (B) Location of tRNA genes (top), predicted secondary structures of tRNAs (middle), and phage and host codon usage comparisons on the tRNA anticodons (bottom). In the bar chart, codons for φEF24C tRNAs are indicated in boldface.
