TABLE 2.
Substrate preferences of the recombinant LnbB
| Sugar | Substratea | Relative activity (%) |
|---|---|---|
| pNP sugars | Galβ1,3GlcNAcβ-pNP | 100b |
| Galβ1,3GalNAcβ-pNP | 30 | |
| GlcNAcβ-pNP | NDc | |
| GalNAcβ-pNP | ND | |
| Galβ1,3GlcNAcα-pNP | ND | |
| Galβ1,3GalNAcα-pNP | ND | |
| PA sugars | ||
| LNT | Galβ1,3GlcNAcβ1,3Galβ1,4Glc-PA | 100d |
| GA1 | Galβ1,3GalNAcβ1,4Galβ1,4Glc-PA | ND |
| LNFP I | Fucα1,2Galβ1,3GlcNAcβ1,3Galβ1,4Glc-PA | ND |
| LNFP II | Galβ1,3(Fucα1,4)GlcNAcβ1,3Galβ1,4Glc-PA | ND |
| LNnT | Galβ1,4GlcNAcβ1,3Galβ1,4Glc-PA | ND |
a
Gal, d-galactose; Glc, d-glucose; Fuc, l-fucose; GalNAc, N-acetyl-d-galactosamine; GlcNAc, N-acetyl-d-glucosamine; LNT, lacto-N-tetraose; GA1, ganglioside GA1; LNFP I, lacto-N-fucopentaose I; LNFP II, lacto-N-fucopentaose II; LNnT, lacto-N-neotetraose.
b
The value obtained with lacto-N-biosyl-β-pNP (250 μM) was taken as 100%.
c
ND, not detectable.
d
The value obtained with LNT-PA (1.0 μM) was taken as 100%.