TABLE 5.
Frequency of carriage of virulence genes in fresh fecal slurry during storage in a manure holding tank
| Gene | Frequency (%) of carriage under the indicated conditionsa
|
||||||
|---|---|---|---|---|---|---|---|
| Wk 0 (n = 184) | Isolation chamber 1
|
Isolation chamber 2
|
Microcosmb
|
||||
| Wk 1 (n = 96) | Wk 7 (n = 93) | Wk 1 (n = 94) | Wk 7 (n = 94) | Wk 1 (n = 94) | Wk 7 (n = 94) | ||
| estB | 1 | 5 ± 5 | |||||
| fedA | 98 | 10 | 13 | 67 ± 27 | |||
| aida-I | 98 | 13 | 13 | 1 | 69 ± 26 | 2 ± 3 | |
| stx2e | |||||||
| astA | 1 | 12 | 11 | 13 | 3 ± 6 | 8 ± 3 | |
| paa | |||||||
| fasA | |||||||
| sepA | |||||||
a
Week 0 represents the initial inoculum. Where no values are given, the frequency of the gene was below the detection level. n, number of isolates.
b
Data from the three laboratory microcosms were pooled and are expressed as the averages ± 1 standard deviation.