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. 2008 May 9;74(13):4133–4143. doi: 10.1128/AEM.00454-08

TABLE 1.

Richness and diversity estimates for bacterial 16S rRNA gene clone libraries from I. strobilina samples collected from the wild and maintained in aquaculture

Sponge (I. strobilina) source (n)a Distanceb Richnessc ACEd Chao1 estimatore Shannon indexf 1/Simpson indexf
Wild (100) 0.2 7 8 7 1.2 2.3
0.03 14 15 14 2.2 8.3
3 Mo of aquaculture (74) 0.2 12 17 17 1.8 4
0.03 41 200 107 3.3 17.8
9 Mo of aquaculture (74) 0.2 14 21 19 1.8 3.8
0.03 37 130 67 3.1 12.8
a

n, number of 16S rRNA gene sequences analyzed.

b

Eighty percent identity was estimated as the phylum-level distance (D = 0.20), and 97% identity was estimated as the species-level distance (D = 0.03).

c

Richness is based on observed unique OTUs.

d

Nonparametric statistical predictions of the total richness of different OTUs were based on the distribution of abundant (>10) and rare (≤10) OTUs.

e

Nonparametric statistical predictions of the total richness of OTUs were based on the distribution of singletons and doubletons.

f

A higher number represents more diversity.