FIG. 4.
Effects of organic solvents on ADHTt. Samples of ADHTt (0.12 mg ml−1) were incubated at 50°C (open and cross-hatched bars) and 60°C (gray and black bars) in the absence and presence of the organic solvents at the indicated concentrations, and assays were performed after 5 h (open and gray bars) and 24 h (cross-hatched and black bars). The activity assays were performed as described in Materials and Methods using ethyl benzoylformate as the substrate. The data obtained in the absence and presence of organic solvents are expressed as percentages of activity relative to the value determined prior to incubation.