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Purification of recombinant ADHTta
| Step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Crude extract | 714 | 12,940 | 18.1 | 100 | 1 |
| Thermal step | 159 | 12,100 | 76.1 | 93.5 | 4.2 |
| DEAE FF | 63 | 9,040 | 143.5 | 69.8 | 7.9 |
| Gel filtration | 54 | 8,159 | 151.1 | 63.0 | 8.3 |
The data are for a 2-liter culture. The substrate was (S)-(−)1-phenylethanol, and the assay temperature was 65°C.
