TABLE 2.

Substrate specificity of ADH_Tt in the oxidation reaction^a

Substrate	Relative activity (%)c
Ethanol	0
1-Propanol	0
2-Propyn-1-ol	0
1-Butanol	0
3-Methyl-1-butanol	0
1-Pentanol	0
1-Hexanol	0
1-Heptanol	0
1-Octanolb	0
2-Propanol	0
(S)-2-Butanol	0
(R)-2-Butanol	0
2,3-Butanediol	0
(S)-2-Pentanol	0
(R)-2-Pentanol	0
3-Pentanol	0
2-Hexanolb	0
2-Heptanolb	0
6-Methyl-5-hepten-2-olb	0
Cyclopentanol	0
Cyclohexanol	13
Cycloheptanol	0
Cyclohexylmethanolb	0
2-Cyclohexylethanol	0
3-Methylcyclohexanolb	0
3-Cyclohexyl-1-propanolb	0
Geraniol	0
Crysanthemyl alcohol	0
Benzyl alcohol	0
2-Methoxybenzyl alcohol	25
3-Methoxybenzyl alcohol	13
4-Methoxybenzyl alcohol	99
4-Methylbenzyl alcohol	0
3-Bromobenzyl alcoholb	0
4-Bromobenzyl alcoholb	0
(R,S)-1-Phenylethanolb	62
(S)-(−)-1-Phenylethanol	100
(R)-(+)-1-Phenylethanol	0
1-(4′-Fluorophenyl)ethanolb	45
1-(4′-Chlorophenyl)ethanolb	26
1-Phenyl-2-propanol	16
(±)-1-Phenyl-1-propanolb	59
(±)-2-Phenyl-1-propanol	0
(R)-1-Phenyl-2-propen-1-ol	14
1-(2-Chlorophenyl)-1-propanolb	0
(R)-(−)-2-Chloro-1-phenylethanol	0
(R,S)-α-(Trifluoromethyl)benzyl alcohol	0
(R)-α-(Trifluoromethyl)benzyl alcohol	0
(S)-α-(Trifluoromethyl)benzyl alcohol	0
trans-Cinnamyl alcoholb	25
Methyl (R)-(−)-mandelate	0
Methyl (S)-(+)-mandelate	0
(R)-(−)-Mandelic acid	0
(S)-(+)-Mandelic acid	0
Ethyl (R)-(−)-mandelate	0
Ethyl (S)-(+)-mandelate	0
(±)-1-Indanold	3,900
(±)-α-Tetralolb	2,700

^aThe activity was measured at 65°C as described in Materials and Methods. The concentration of each substrate was 20 mM.

^bThe substrate was dissolved in 50% 2-propanol (the only exception was α-tetralol, which was dissolved in 100% 2-propanol).

^cRelative rates were calculated by defining the activity for (S)-(−)-1-phenylethanol as 100%.

^dThe substrate was dissolved in 100% acetonitrile.