TABLE 3.

Substrate specificity of ADH_Tt in the reduction reaction

Substratea	Relative activity (%)b
Ketones
3,3-Dimethyl-2-butanone	0
2-Pentanone	0
3-Methyl-2-pentanone	0
4-Methyl-2-pentanone	0
2-Methyl-3-pentanone	0
5-Chloro-2-pentanone	0
2,5-Hexanedione	0
6-Methyl-5-hepten-2-one	0
Cyclopentanone	0
2-Methylcyclopentanone	0
Cyclohexanone	0
2-Methylcyclohexanone	0
3-Methylcyclohexanone	0
4-Methylcyclohexanone	0
2,6-Dimethylcyclohexanone	0
Cycloheptanone	0
(±)-Camphor	0
4-Methoxyphenylacetone	0
2-Acetylfuran	0
Phenylacetone	0
Acetophenone	0
2-Hydroxyacetophenone	0
3′-Methoxyacetophenone	0
4′-Methoxyacetophenone	0
2,2,2-Trifluoroacetophenone	100
2,2-Dichloroacetophenone	32
3-Chloropropiophenone	0
4-Chlorobutyrophenone	10
2′,3′,4′,5′,6′-Pentafluoroacetophenone	0
Chalcone	0
Benzil	0
1-Phenyl-1,2-propanedione	146
1-Indanone	3
α-Tetralone	4
Aldehydes
Propionaldehyde	0
Butyraldehyde	0
iso-Butyraldehyde	0
3-Methyl-butyraldehyde	0
Valeraldehyde	0
Hexanal	0
Benzaldehyde	14
Salicylaldehyde	0
3-Hydroxybenzaldehyde	0
4-Hydroxybenzaldehyde	0
2-Methoxybenzaldehyde	13
3-Methoxybenzaldehyde	14
4-Methoxybenzaldehyde	13
trans-Cinnamaldehyde	0
Keto esters
Ethyl pyruvate	0
Ethyl 3-oxohexanoate	0
MBF	57
Ethyl benzoylformate	100
Ethyl benzoylacetate	0
Keto acids
Phenylglyoxylic acid	0
Sodium phenylpyruvate	0

^aThe activity was measured at 65°C as described in Materials and Methods. Substrates were dissolved in 2-propanol and added to the reaction mixture (5 mM in 2% [vol/vol] 2-propanol).

^bThe percentages were determined by comparison to 2,2,2-trifluoroacetophenone for ketones and aldehydes and to ethyl benzoylformate for keto esters and keto acids.