TABLE 1.
Plasmids used and constructed in this study
| Plasmid | Relevant features | Copy no. | Reference or source |
|---|---|---|---|
| Used | |||
| pAKE600 | oriMB1oriTRK2 PnrsacB | High | 7 |
| pBGS18 | oriMB1 Kmr | High | 36 |
| pET28 | oriMB1 Kmr T7p lacO His6 tag, T7 tag | Medium | Novagen |
| pET28mod | oriMB1 Kmr T7p lacO His6 tag, T7 tag deleted, no BamHI site | Medium | G. Jagura-Burdzy |
| pGBT30 | oriMB1 PnrlacIqtacp expression vector | High | 17 |
| pGEM-T Easy | oriMB1Pnr | High | Promega |
| pPTOI | oriSC101 Kmr promotorless xylE | Medium | 39 |
| pUC18 | oriMB1 Pnr | High | 43 |
| RA3 | IncU Cmr Smr Sur | Low | F. Hayes |
| Constructed | |||
| pAKB1.101 | pGEM-T with PCR product (6L2 and str7)a corresponding to 3,155-bp fragment of RA3 | ||
| pAKB1.102 | pGEM-T with PCR product (inp5 and str7) corresponding to 1,261-bp fragment of RA3 | ||
| pAKB1.103 | pGEM-T with PCR product (rep2 and str7) corresponding to 1,861-bp fragment of RA3 | ||
| pAKB7.5 | pBGS18 with 460-bp HincII-BamHI fragment from RA3 (coordinates 9395-9854) | ||
| pAKB9.5 | pAKE600 with BamHI-PstI fragment from pAKB7.5 | ||
| pMOB1.4 | pBGS18 with 2,563-bp PCR product (miniR1 and snaB2) | ||
| pMOB1.5 | pBGS18 with 327-bp PCR product (ant3 and ant2) | ||
| pMOB1.5.1 | pGBT30 with SacI-SalI fragment of pMOB1.5 to create transcriptional fusion tacp-repA | ||
| pMOB1.6 | pBGS18 with 1,409-bp PCR product (rep3 and rep4) | ||
| pMOB1.6.1 | pGBT30 with SacI-SalI fragment of pMOB1.6 to create transcriptional fusion tacp-repB | ||
| pMOB1.7 | pBGS18 with 362-bp PCR product (rep5 and rep7) | ||
| pMOB1.7.1 | pPTO1 with SphI-BamHI fragment of pMOB1.7 to create transcriptional fusion repBp1/p2-xylE | ||
| pMOB1.8 | pBGS18 with 312-bp PCR product (rep6 and rep7) | ||
| pMOB1.8.1 | pPTO1 with SphI-BamHI fragment of pMOB1.8 to create transcriptional fusion repBp2-xylE | ||
| pMOB1.9 | pBGS18 with 362-bp PCR product (rep1 and rep2) | ||
| pMOB1.9.1 | pPTO1 with SphI-BamHI fragment of pMOB1.9 to create transcriptional fusion repXp-xylE | ||
| pMOB1.10 | pBGS18 with 376-bp PCR product (ant1 and ant2) | ||
| pMOB1.10.1 | pPTO1 with BamHI fragment of pMOB1.10 to create transcriptional fusion repAp-xylE |
a
Primers used in PCR.