TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| S17-1 | thi-1 proA hsdR17 (rK− mK+) recA1; tra genes of plasmid RP4 integrated into the genome | 52 |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 (rK− mK+) supE44 relA1 λ−lac [F′ proAB lacIqlacZΔM15 Tn10 (Tcr)] | 10 |
| Tetrathiobacter mimigardefordensis (DSM 17166T; LMG 22922T) | Wild type, DTDP-degrading organism | 66 |
| Plasmids | ||
| pSUP5011 | Apr Cmr Kmr Tn5::mob | 51 |
| pBluescript SK (−) | AprlacPOZ′ | Stratagene, San Diego, CA |
a
For abbreviations used in genotypes of E. coli, see reference 6.