TABLE 2.
Genotypic characterization of Tn5::mob-induced mutants of T. mimigardefordensis defective in the utilization of DTDP
| Mutant | Phenotype regarding DTDP | Insertion locus of Tn5::mob (gene product)a | % Amino acid identity (strain) |
|---|---|---|---|
| Jhw51c | Negative | lpdA (dihydrolipoamide dehydrogenase) | 79 (Bordetella pertussis TohamaI) |
| Jhw90 | Negative | lpdA (dihydrolipoamide dehydrogenase) | 79 (Bordetella pertussis TohamaI) |
| Jhw101 | Negative | lpdA (dihydrolipoamide dehydrogenase) | 79 (Bordetella pertussis TohamaI) |
| KK14 | Negative | cdo (cysteine dioxygenase type I) | 65 (Verminephrobacter eiseniae EF01) |
| KK15 | Negative | cdo (cysteine dioxygenase type I) | 65 (Verminephrobacter eiseniae EF01) |
| Jhw17 | Negative | cdo (cysteine dioxygenase type I) | 65 (Verminephrobacter eiseniae EF01) |
| Jhw103 | Negative | cdo (cysteine dioxygenase type I) | 65 (Verminephrobacter eiseniae EF01) |
| JhwA8/121 | Negative | sucC (succinyl-CoA synthetase, beta chain) | 93 (Bordetella pertussis TohamaI) |
| Jhw38 | Leaky | sucC* (succinyl-CoA synthetase, beta chain) | 93 (Bordetella pertussis TohamaI) |
| JhwAA14 | Leaky | acnB (putative bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase) | 86 (Burkholderia sp. strain 383) |
| Jhw13b | Negative | acnB (putative bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase) | 86 (Burkholderia sp. strain 383) |
| KK13 | Leaky | lonA (ATP-dependent Lon protease) | 39 (Bacillus clausii KSM-K16) |
| JhwI | Negative | MOSC domain* (sulfur carrier) | 44(Bordetella bronchiseptica RB50) |
| JhwV | Negative | dnaK (chaperone protein) | 88 (Bordetella bronchiseptica RB50) |
| JhwIX | Leaky | dnaJ (molecular chaperone) | 80 (Bordetella avium 197N) |
| JhwX | Leaky | dnaJ (molecular chaperone) | 80 (Bordetella avium 197N) |
a
*, closest specified gene identified adjacent to the respective Tn5::mob insertion locus.