TABLE 1.
Sample | Distance from landfill (m) | No. of clones analyzed | No. of ARDRA types | Coverage (%) | Aerobic MPN | Anaerobic MPN | MPN-PCR |
---|---|---|---|---|---|---|---|
P1-3m | 21 | 33 | 17 | 66 | 450 | 558 | 1,860 |
P1-5m | 21 | 36 | 4 | 97 | NDb | ND | 760 |
P2-3m | 30 | 43 | 13 | 81 | ND | ND | 3,000 |
P4-3m | 48 | 37 | 9 | 95 | 1,260 | 558 | 86 |
P6-3m | 68 | 42 | 4 | 98 | ND | ND | 1,860 |
C1-3m | Nonpolluted | 32 | 4 | 97 | 138 | 138 | 860 |
See Fig. 1 for locations. The numbers of clones and unique ARDRA types and the coverage were derived from analysis of clone libraries. MPN estimates were obtained by culturing (eukaryotic cells/g sediment) and by PCR-based, culture-independent MPN analysis (18S rRNA gene copies/g sediment).
ND, not determined.