TABLE 1.

Primers used to generate constructs for NoV capsid expression system and real-time PCR standard curve plasmids in this study

Group and genotype	Primer	Sequence (5′ to 3′)	Polaritya	Restriction enzyme site	Locationd
NoV capsid expression vectors
GI-1	GIF	GAA TTC ATG ATG ATG GCG TCT AAG G	+	EcoRI	5358-5376
	GIR	GCG GCC GCC AGT GTG ATG GAT A	−	NotI	TOPO vectore
GII-4	GIIF	CAT ATG AAG ATG GCG TCG AGT G	+	NdeI	5058-5103
	GIIR	TAA ACT GGA AGG AAG CTT GAT G	−	HindIII	6827-6842
Real-time PCR standard curve plasmids
GI-1	GI SCCF	TGG ARA TGT ATG TCC CAG G	+		5256-5274
	GI SCCR	CCA ACC CAR CCA TTR TAC Ab	−		5653-5671
GII-4	GII SCCF	RGC TNT NGA AAT NAT GGTc	+		4338-4355
	GII SCCR	CCR CCN GCA TRH CCR TTR TAC ATb	−		5367-5389

^a+, sense primer; −, antisense primer.

^bSee reference 17.

^cModified nucleotides from reference 18.

^dNucleotide positions based on the Norwalk virus (GI, accession no. M87661) and Lordsdale virus (GII, accession no. X86557).

^epCR2.1-TOPO vector (Invitrogen).