FIG. 3.

Effects of protease and hemolysin inhibitors on proteolytic and hemolytic activities and on toxicity to Pacific oyster larvae. Enzymatic activities and toxicity levels are shown as percentages of those for nontreated samples. For the toxicity assay, filter-sterilized supernatants were added to a final concentration of 1%. The error bars indicate standard deviations (n = 3). Data for proteolytic and hemolytic activities were evaluated by Student's t test (*, P < 0.01 compared with the nontreated control). Data for larval mortality were evaluated by chi-square test (*, P < 0.05 compared with nontreated control). NT, nontreated; TEP, tetraethylene pentamine; OPA, 1,10-phenanthroline; PMSF, phenylmethylsulfonyl fluoride; PPA, pepstatin A; CHOL, cholesterol.