TABLE 1.
Primers used in this study
| Primer and use | Sequencea (5′ to 3′) |
|---|---|
| Initial identification of vtpA and vthA | |
| HA5′deg1 | GGNCCNGGNGGNAAYCARAARAC |
| HA3′4 | GCCCGCTTTGACATACAGATCCGCATCGCCC |
| Cy5′2 | CGCGTCAATGTGGCACAAATGCGCTCGGTACAATC |
| Cy3′2 | TAGGCCCCAAACTTGGTTCCAACTGCCGTGACAGC |
| Inverse PCR | |
| 5HapOut | GTCCGTACCGAAGTTGTATTG |
| 3HapOut | GACAGCAAACAGCACGTTTGA |
| 5CytOut | GCCAATGTACGTGCGGCGGTTCGCCCAGCTTTGG |
| 3CytOut | GCAACGACAGGATGGTCAATTCCACGTACGGTTCG |
| pBAD expression cloning | |
| 5hapexp | GAGGAATAATAAATGAAACAACGTCAAATGCTTTG |
| 3hapexp | TTAGTCTAATCTTAGTGTCACGC |
| 5cytexp | GAGGAATAATAAATGAAAACTTCTACAATTTTCAC |
| 3cytexp | TTAAAGTTTGATTTGCTGTAGTG |
| vtpA internal deletion | |
| 5HapDel | GGGGGGCGGCCGCCAATACAACTTCGGTACGGAC |
| 3HapDel | GGGGGACTAGTTCAAACGTGCTGTTTGCTGTC |
a
N = A, G, C, or T; Y = C or T; R = A or G.