TABLE 3.

Expression of nodY-lacZ fusion in response to the addition of supernatants from B. japonicum USDA110 cultures grown to various population densities in complex or minimal medium

OD600 of culture	β-Galactosidase activity ± SD (units) with supernatant from:
	Complex mediuma	Minimal mediumb
0.08	126 ± 0.5	140 ± 22.2
0.20	112 ± 5.5	132 ± 2.2
0.50	105 ± 1.3	128 ± 1.0
1.00	72 ± 4.7	82 ± 2.3
1.50	71 ± 4.2	82 ± 1.8
2.00	59 ± 5.0	78 ± 3.6
2.50	45 ± 6.5	52 ± 4.7
Uninducedc	22 ± 4.4	21 ± 0.6

^aArabinose-gluconate (AG) medium (28) plus yeast extract.

^bBergersen's defined minimal medium (2).

^cUninduced cultures (no genistein) were grown to an OD₆₀₀ of 0.2.