TABLE 6.
Inoculant strain | % Nodule occupancy by strain:
|
Total no. of nodules | ||
---|---|---|---|---|
USDA110 | USDA123 | Both | ||
USDA110 alone | 100 A | 0 E | 0 B | 20.3 F |
110-BjB3 (nolA mutant) alone | 100 A | 0 E | 0 B | 28.0 BC |
110-JD21 (nodD2 mutant) alone | 100 A | 0 E | 0 B | 28.3 BCD |
USDA123 alone | 0 D | 100 A | 0 B | 25.3 CDE |
USDA110 and USDA123 | 20.0 C | 30.0 BC | 50.0 A | 24.7 E |
110-BjB3 and USDA123 | 21.7 C | 40.0 B | 38.3 A | 30.7 B |
110-JD21 and USDA123 | 46.7 B | 16.7 D | 36.6 A | 34.7 A |
Plants were inoculated with 109 cells/ml of each bacterium (1:1 ratio) and grown in a vermiculite-perlite mixture (3:1) in sterile Leonard jar assemblies. Values are means of results for ≥50% of total number of nodules. Means within a column not followed by the same letter differ significantly at a P value of 0.05 as tested by ANOVA and the Duncan-Waller multiple-range test.