TABLE 2.
Putative genes in E. sakazakii O:1 or O:2 O-antigen gene cluster
| ORF | Location in sequence (bp) | G+C content (%) | Size (aaa) | Similar protein | Source (accession no.) | % Identical/% similar (no. of aa) | Putative function |
|---|---|---|---|---|---|---|---|
| O:1 cluster | |||||||
| orf1 | 1-891 | 53.4 | 296 | GalF | Salmonella enterica (AE017220) | 87/95 (295) | UDP-glucose pyrophosphorylase |
| orf2 | 1282-2361 | 50.3 | 282 | RfbB | Shigella flexnerii (NP_707936) | 87/93 (273) | dTDP-d-glucose 4,6 dehydratase |
| orf3 | 2358-3230 | 40.9 | 290 | RmlA | Escherichia coli (AAT77170) | 81/90 (287) | dTDP-glucose pyrophosphorylase |
| orf4 | 3232-3633 | 35.8 | 133 | WbtA | Escherichia coli (ABK27315) | 55/73 (131) | Putative isomerase |
| orf5 | 3626-4078 | 31.8 | 150 | FdtC | Escherichia coli (AAT77171) | 49/58 (147) | Acetyltransferase |
| orf6 | 4079-5014 | 34.4 | 311 | Xanthomonas axonopodis pv. Citri (NP_642021) | 45/62 (306) | Hypothetical protein | |
| orf7 | 5011-6114 | 36.7 | 367 | FdtB | Escherichia coli (AAT77174) | 58/73 (365) | Aminotransferase |
| orf8 | 6111-7373 | 37.3 | 420 | Wzx | Escherichia coli (AAT77175) | 46/67 (413) | O-antigen flippase |
| orf9 | 7373-8317 | 32.5 | 314 | WbtE | Escherichia coli (AAS73170) | 28/50 (254) | Putative glycosyltransferase |
| orf10 | 8356-9636 | 31.9 | 426 | Wzy | Escherichia coli (AAZ85713) | 19/43 (272) | O-antigen polymerase |
| orf11 | 9623-10501 | 36.7 | 292 | WbgQ | Shigella boydii (AAL27350) | 32/51 (281) | Putative glycosyltransferase |
| orf12 | 10491-11255 | 34.0 | 254 | Shewanella baltica (ZP_01435692) | 55/68 (243) | Glycosyltransferase | |
| orf13 | 11469-12875 | 54.3 | 468 | Gnd | Escherichia coli (ZP_00926655) | 93/97 (469) | 6-Phosphogluconate dehydrogenase |
| O:2 cluster | |||||||
| orf1 | 1-594 | 55.1 | 197 | GalF | Escherichia coli (AAS99158) | 83/90 (197) | UDP-glucose pyrophosphorylase |
| orf2 | 986-2068 | 50.3 | 360 | RmlB | Escherichia coli (ABI98981) | 84/91 (360) | dTDP-glucose 4,6-dehydratase |
| orf3 | 2065-2967 | 49.5 | 300 | RmlD | Shigella boydii (AAY23334) | 80/88 (298) | dTDP-4-dehydrorhamnose reductase |
| orf4 | 3017-3895 | 46.6 | 292 | RmlA | Salmonella enterica (P55254) | 91/96 (292) | dTDP-glucose synthase |
| orf5 | 3899-4441 | 35.9 | 180 | RfbC | Shigella boydii (YP_407363) | 81/90 (180) | dTDP-4-dehydrorhamnose 3,5-epimerase |
| orf6 | 4488-5744 | 30.2 | 418 | RfbX | Escherichia coli (NP_416541) | 27/52 (406) | Putative O-antigen transporter |
| orf7 | 5741-6718 | 33.2 | 325 | Escherichia coli (ZP_01699967) | 22/42 (280) | Glycosyltransferase | |
| orf8 | 6730-7485 | 29.4 | 251 | WbsV | Shigella boydii (AAS98030) | 30/48 (241) | Glycosyltransferase |
| orf9 | 7475-8473 | 31.0 | 332 | Wzy | Shigella boydii (AAS98031) | 27/43 (317) | O-antigen polymerase |
| orf10 | 8493-9332 | 28.9 | 279 | WbsW | Shigella boydii (AAS98032) | 30/47 (268) | Glycosyltransferase |
| orf11 | 9334-10341 | 31.9 | 335 | WbgM | Escherichia coli (YP_541302) | 30/46 (110) | Putative galactosyltransferase |
| orf12 | 10331-11095 | 29.9 | 254 | WbbL | Escherichia coli (P36667) | 40/58 (257) | Putative lipopolysaccharide biosynthesis glycosyltransferase |
| orf13 | 11462-12868 | 54.4 | 468 | Gnd | Escherichia coli (ZP_00926655) | 93/97 (469) | 6-Phosphogluconate dehydrogenase |
a
aa, amino acids.