FIG. 3.

RT-PCR amplification of dfdA mRNA from Rhodococcus sp. strain HA01 grown on DBF (A) or fructose (B). M lanes contain the molecular size marker Hyperladder 1 (Bioline). cDNA generated from 1 μg template RNA was serially diluted (3.2-fold) with nuclease-free water, and 1 μl of each dilution was subjected to amplification by PCR (lanes 1 to 15). Negative controls included undiluted RT-PCR mixtures devoid of reverse transcriptase (lanes 1 to 4 in panel C) or template cDNA (lane 5 in panel C). PCR mixtures containing 1 ng of genomic DNA as a template were used as positive control (lane 6 in panel C) in the same experiment.