TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Bacterial strains | ||
| Sphingomonas wittichii RW1 | Dibenzofuran-utilizing bacterium | DSMZa |
| Rhodococcus sp. strain ATCC 12674 | Bacterium incapable of utilizing dibenzofuran | DSMZb |
| Rhodococcus sp. strain HA01 | Dibenzofuran-utilizing bacterium | This study |
| E. coli BL21(DE3)(pT7-5RW) | pT7-5 with 1.4-kb PstI/SalI insert carrying dbfB gene of S. wittichii RW1 | 18 |
| E. coli JM109 | recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) e14− (mcrA) supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+ lacIqlacZΔM15] | Stratagene |
| Plasmids | ||
| pGEM-T Easy | Cloning vector | Promega |
| pUC119 | AprlacZ; pMB9 replicon | Takara Shuzo Co. |
| pRSG43 | E. coli-Rhodococcus shuttle vector; Rhodococcus rhodochrous cryptic plasmid pRC4 integrated into the AflIII site of pHSG299 | Masahiro Takeo |
| pDFDE | Apr; pUC119 with 4.4-kb EcoRI/PstI insert carrying dfdA1A2A3A4 genes of strain HA01 | This study |
| pDFDR | Kmr; pRSG43 with 4.4-kb EcoRI/PstI insert carrying dfdA1A2A3A4 genes of strain HA01 | This study |
| pDBFA12a | Apr; pUC119 with 3-kb HindIII/EcoRI insert carrying dbfA1A2 genes of strain HA01 | This study |
| pDBFA12 | Kmr; pRSG43 with 3-kb XbaI/EcoRI insert carrying dbfA1A2 genes of strain HA01 | This study |
a
DSMZ, Deutsche Sammlung von Mikoorganismen und Zellkulturen, Braunschweig, Germany. This strain was DSMZ 6014 in the DSMZ culture collection.
b
This strain was DSMZ 43287 in the DSMZ culture collection.