TABLE 5.

Prediction of ethanol titers of TCS083/pLOI297 and CT1101/pLOI297^a

Strain	Substrate(s)	Titer of biomass (g/liter)	w1c (%)	w2c (%)	Predicted amt of ethanolb (g/liter)	Amt of ethanol measured (g/liter)
TCS083/pLOI297	Glucose	3.11 ± 0.47	16 ± 2	84 ± 2	39.20 ± 0.34	38.77 ± 0.63
TCS083/pLOI297	Xylose	3.25 ± 0.12	42 ± 1	58 ± 1	39.22 ± 0.09	39.07 ± 0.59
TCS083/pLOI297	Glucose and xylose	3.54 ± 0.16	32 ± 1	68 ± 1	39.46 ± 0.57	38.81 ± 0.91
CT1101/pLOI297	Glucose and xylose	1.48 ± 0.02	39 ± 1	61 ± 1	19.32 ± 0.73	20.03 ± 0.18

^aPredictions are based on the overall stoichiometric equations of the efficient ethanol-producing pathways (Table 1). The values are reported as means ± standard deviations of six independent measurements from duplicate runs of batch bioreactors.

^bThe predicted amount of ethanol can be calculated as follows: , where [ETOH]predicted (g/liter) is the ethanol titer predicted, [sugar] (g/g) is the amount of sugar consumed, and Y_ETOH/sugar (g ethanol/g sugar) is the theoretical yield of ethanol on sugar calculated by using stoichiometric equations. Subscripts “1” and “2” refer to the growth-associated phase and non-growth-associated phase, respectively.

^cw₁ and w₂ are the weighting factors of glucose (xylose) channeled into either the growth-associated ethanol-producing pathway or the non-growth-associated ethanol-producing pathway, respectively. w₁ can be calculated as follows: w₁ = 100% × [sugar]₁/[sugar], and w₂ = 100% − w₁. [sugar]₁ can be determined by the following formula: , where X (g/liter) is the experimentally determined biomass and (g biomass/g sugar) is the yield of biomass on sugar calculated by using stoichiometric equations.