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Experimental setup and sampling of the SSMS for inducing the growth of hard-to-culture mercury-resistant soil bacteria. Subsamples of 100 μl (20 μl for membranes to be incubated for 21 to 28 days) of the soil extract were diluted with 10 ml salt buffer before being filtered onto polycarbonate membranes. PBS, phosphate-buffered saline.
