TABLE 2.

Distribution over time of the 25 different 16S rRNA gene sequences obtained from cultivable bacteria following SSMS preincubation

Isolate or parameter	Bacterial phyluma	Sampleb from 35-55 cm on day:				Sampleb from 80-100 cm on day:
		7	14	21	28	7	14	21	28
SEQ 1	CFB						+	+	+
SEQ 2	CFB							+	+
SEQ 3	CFB							−
SEQ 4	Betaproteobacteria	+		+	+			+	+
SEQ 5	Betaproteobacteria	+	+	+	+		+	+	+
SEQ 6	Betaproteobacteria				+			+
SEQ 7	Betaproteobacteria		−
SEQ 8	Betaproteobacteria							−
SEQ 9	Betaproteobacteria	+			+
SEQ 10	Betaproteobacteria				−
SEQ 11	Betaproteobacteria			−
SEQ 12	Betaproteobacteria			+	+		+
SEQ 13	Betaproteobacteria				+		+	+
SEQ 14	Betaproteobacteria	+		+	+		+	+	+
SEQ 15	Betaproteobacteria	−
SEQ 16	Betaproteobacteria				−
SEQ 17	Betaproteobacteria		+	+		+	+
SEQ 18	Gammaproteobacteria							−
SEQ 19	Gammaproteobacteria					+			+
SEQ 20	Gammaproteobacteria					−
SEQ 21	Alphaproteobacteria								−
SEQ 22	Firmicutes				−
SEQ 23	Actinobacteria	+		+	+		+	+
SEQ 24	Actinobacteria	+			+
SEQ 25	Actinobacteria	+		+
No. of sequences		8	3	8	12	3	7	11	7
No. of 1st appearances		8	2	2	5	3	6	6	1

^aCFB, Cytophaga-Flavobacterium-Bacteroidetes. Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria are subphyla of Proteobacteria.

^b+, presence of a specific sequence; −, unique sequence not seen at another sampling time.