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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Protein Expr Purif. 2008 Mar 23;59(2):215–222. doi: 10.1016/j.pep.2008.01.023

Fig. 2.

Fig. 2

Electrophoretic profile of total cellular proteins obtained from E. coli DE3 strains BL21, Rosetta-2, C41, and C43 (labeled above each lane) expressing TM4-Cx43CT after IPTG induction for 4 hrs (panel A, lanes 3, 5, 7, and 9). Lanes 2, 4, 6 and 8 are controls (no IPTG). Lane 1 contains the protein molecular weight marker. B) The presence of the TM4-Cx43CT polypeptide was confirmed by western blot analysis using a Zymed Laboratories Inc. polyclonal anti-Cx43CT antibody. The blots correspond to lanes 6 and 7 from panel A. The arrows represent the location of the TM4-Cx43CT monomer. The asterisk represents TM4-Cx43CT dimers.