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. 2008 Apr 2;15(6):925–931. doi: 10.1128/CVI.00500-07

FIG. 7.

FIG. 7.

Expression and function of anti-CR1 scFv in S. gordonii. (A) Immunoblot of culture supernatant from S. gordonii SecCR1 (lane R) or parent S. gordonii (lane P) concentrated 20-fold by trichloroacetic acid precipitation and anti-CR1 scFv isolated by NiCAM chromatography. Lanes 1 to 4 are fractions from the column. (B) Eluted (bars 1, 2, 3, and 4) and unbound (Unbd) fractions from the NiCAM column were tested for binding to dendritic cells in ELISA. PBST, wells in which PBST was added in place of fractions. The results shown are means ± standard deviations of triplicate wells.