FIG. 1.

ssRNA-DCs and LPS-DCs display a mature phenotype. Monocyte-derived DCs were differentiated in the presence or not (cDCs) of LPS (LPS-DCs) or ssRNA/Dotap (ssRNA-DCs). On day 5, DCs were kept for 24 h in medium (filled histograms) or stimulated with LPS (thick lines). Expression of surface HLA-DR, CD1a, CD14, CD40, CD80, CD83, and CD86 was determined by FACS analysis. The background signal of an Ig isotypic control is shown (dotted lines). The phenotype of DCs differentiated in the presence of Dotap did not differ from that of cDCs (not shown).