FIG. 3.

ssRNA-DCs and LPS-DCs have a reduced Th-polarizing capacity. Allogeneic naive CD4 T cells were stimulated for 6 days with the different DC preparations: cDCs, LPS-DCs, and ssRNA-DCs not receiving or receiving a late LPS maturation stimulus. Control cells were also stimulated with ssRNA. The phenotype of activated T cells was determined by FACS analysis after stimulation with PMA-ionomycin and intracellular staining of IFN-γ and IL-4. Results are shown as dot plots (A) or bar histograms (B) representative of experiments with different donors. Compared to cDCs, LPS-DCs and ssRNA-DCs receiving a late TLR4-mediated signal induced significantly fewer IFN-γ-producing cells (indicated with asterisks [P < 0.05]).