FIG. 1.

Expression and purification of M. tuberculosis proteins Rv1168c (A) and Hsp60 (B). The recombinant protein was expressed in strain BL21 of Escherichia coli and was purified to homogeneity using the Ni-nitrilotriacetic acid protein purification kit. Results shown are for Coomassie blue-stained sodium dodecyl sulfate gels showing uninduced (UN) and induced (IN) cell lysates, a protein molecular size marker (M), and different lanes showing different elution fractions containing purified protein (lanes 1 to 7 in panel A and lanes 1 to 5 in panel B) obtained during purification of the respective proteins. The arrow on the right indicates the position of the Rv1168c protein (∼42 kDa) or Hsp60 protein (∼60 kDa).