TABLE 2.
Rv1168c mounts stronger T-cell responses in TB patients than in BCG-vaccinated controlsa
| Antigen | Controls
|
Pulmonary TB patients
|
Extrapulmonary TB patients
|
|||
|---|---|---|---|---|---|---|
| IFN-γ (pg/ml) | IL-5 (pg/ml) | IFN-γ (pg/ml) | IL-5 (pg/ml) | IFN-γ (pg/ml) | IL-5 (pg/ml) | |
| Rv1168c | 92 ± 24 | 80 ± 22 | 287 ± 70 | 147 ± 69 | 325 ± 125 | 191 ± 61 |
| PPD | 91 ± 17 | 78 ± 19 | 177 ± 52 | 81 ± 31 | 141 ± 51 | 106 ± 50 |
a
PBMCs collected from TB patients and BCG-vaccinated controls were stimulated in triplicate with 3 μg/ml of Rv1168c or 10 μg/ml PPD. After 4 days, levels of IFN-γ and IL-5 cytokines secreted in the culture supernatants were estimated by EIA.