FIG. 2.

Relationship of ODs to numbers of parasites plotted on a log versus log scale of results from five assay repetitions using washed iRBCs diluted in uninfected whole blood. The upper LOQ measured is 750 iRBCs/μl, which corresponds to an OD of 1.97. The lower LOD is 5.86 iRBCs/μl (inset table, horizontal arrow), while the lower LOQ, i.e., the point at which the analysis becomes linear and remains reproducible, is 11.7 iRBCs/μl (vertical arrow). The inset graph shows the quantities of PfHRP2 in spiked samples.