Cell death following LeTx exposure. Average percentages of trypan blue-positive cells are shown. C, cells alone; C+S, cells incubated with serum; C+T, cells incubated with toxin; C+S+T, cells incubated with serum and toxin; C+HI+T, cells treated with heat-inactivated serum and toxin. Bars represent 1 standard deviation. *, P value of <0.05 for comparison between C+T and C+S+T values.