TABLE 2.
Percentage of naïve CD4+ T cells expressing CD31 in peripheral blood, freshly isolated PBMCs, and cryopreserved PBMCs and absolute differences in percentages in PBMCs or cryopreserved PBMCs from that in peripheral blooda
| Cell source | Mean % (IQR) naïve CD4+ T cells expressing CD31 in:
|
Difference (IQR) between % of CD31-expressing naïve CD4+ T cells in PB and that inb:
|
P value Cryopreserved PBMCs
|
||||
|---|---|---|---|---|---|---|---|
| PB | PBMCs | Cryopreserved PBMCs | PBMCs | Cryopreserved PBMCs | PB vs freshly isolated PBMCs | PB vs cryopreserved PBMCs | |
| HIV− subjects (n = 17) | 51.5 (44-57) | 50.7 (43-58) | 54.2 (44-65) | 0.8 (−1.0 to 4.0) | −2.7 (−8.0 to 0.0) | 0.5 | 0.18 |
| HIV+ subjects (n = 37) | 49.5 (41-57) | 47.7 (34-61) | 54.8 (41-68) | 1.8 (−3.0 to 7.0) | −5.3 (−11.0 to −1.0) | 0.4 | 0.003 |
a
PB, peripheral blood.
b
Values are absolute differences of peripheral blood (tested at <4 h after collection) from PBMCs or cryopreserved PBMCs.
c P values from paired t test comparing peripheral blood (tested at <4 h after collection) and PBMCs or cryopreserved PBMCs.