TABLE 1.

Primers used for the amplification of the gp19, gp36, gp140, and gp200 genes from the US, BR, and IS E. canis strains

Gene	Forward primer	Sequence of forward primer (5′-3′)	Reverse primer	Sequence of reverse primer (5′-3′)	Amplicon size (bp)
p19	p19F	AAAATTAGTGTTGTGGTTATG	p19R	TTTTACGCTTGCTGAAT	492
p36	p36F-L	GGAATGATTTATTAAAAAAGTTTGAC	p36R-L	GATCGTTGGATGTTGG	2,080
p140	p140F	ATGGATATTGATAACAATAATGTGACTAC	p140R	TATTAAATCAACTGTTTCTTTGTTAGT	2,061
p200	p200F	TTTGCCATTCAGGAACATCG	p200R	TGCACCTCATATCCAACTAGAAACAC	4,597