TABLE 3.
Reactivities of mouse sera with PA synthetic peptides
| Mouse serum | Reactivity with the following synthetic peptidea:
|
Neutralizationc | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PA26-29 | PA50-61 | PA91-114 | PA121-150 | PA143-158 | PA339-359 | PA421-440 | PA509-516 | PA645-657 | PA678-697 | PA711-715 | Positive controlb | ||
| A/J PA1 | − | − | − | − | − | − | − | − | − | − | − | + | No |
| A/J PA4 | − | − | − | − | − | − | − | − | − | + | + | + | No |
| A/J PA2-4 | − | − | − | − | − | − | − | − | − | − | − | + | No |
| A/J PA1-4 | − | − | − | − | − | − | − | − | − | − | − | + | Yes |
| BALB/c PA1 | − | − | − | + | − | − | − | − | − | − | − | +++ | No |
| BALB/c PA4 | − | − | − | − | − | − | − | − | − | − | − | + | No |
| BALB/c PA2-4 | − | − | − | − | − | − | − | − | − | − | − | + | No |
| BALB/c PA1-4 | + | + | − | +++ | ++ | ++ | +++ | − | − | ++ | − | +++ | Yes |
| C57BL/6 PA1 | − | − | − | +++ | − | − | − | − | − | − | − | +++ | Yes |
| C57BL/6 PA4 | − | − | − | − | − | − | − | − | + | + | + | + | No |
| C57BL/6 PA2-4 | − | − | − | − | − | − | − | − | − | − | − | + | No |
| C57BL/6 PA1-4 | + | − | − | ++ | − | − | − | − | − | − | − | +++ | Yes |
| Swiss Webster PA1 | + | − | − | − | − | − | − | − | − | − | − | + | No |
| Swiss Webster PA4 | − | − | − | − | − | − | − | − | + | + | − | + | No |
| Swiss Webster PA2-4 | − | − | − | − | − | − | − | − | − | − | − | + | No |
| Swiss Webster PA1-4 | − | − | − | − | − | − | − | − | − | − | − | + | Yes |
a
Plus and minus signs indicate the relative reactivities of the various immune sera for the peptide as measured by ELISA, ranging from none (−) to weak (+), moderate (++), and strong (+++).
b
The relative reactivities of the various immune sera to PA were measured by ELISA, as described in Materials and Methods.
c
Neutralization capacity of anti-PA antibodies in immune sera to protect cells against LeTx in vitro (from Fig. 2).