FIG. 1.

Sensitivity of the wild-type strain, representative single mutants ssk1Δ and skn7Δ, double mutant ssk1Δ skn7Δ, and reintegrant strains ssk1Δ+SSK1 and skn7Δ+SKN7 to different stresses. All strains were grown overnight in YPD liquid medium, the cells were counted, and then dilutions (10⁵ to 10² cells) were spotted onto YPD plate supplemented or not (control) with 1.5 M sorbitol, NaCl or KCl, 6 mM of H₂O₂, 25 mM methyglyoxal, 2 μg ml⁻¹ fludioxonil, or 25 μg ml⁻¹ iprodione. To test UV sensitivity, cells on YPD plates were exposed to UV for 12 s (2,880 J m⁻²). Each plate was further incubated for 2 days at 35°C and photographed. For high-temperature sensitivity, spotted cells were incubated for 1 day at 43°C and photographed. This experiment was done in triplicate, and pictures show representative plates for each condition. WT, wild type.