FIG. 1.

Ectopic integration of A. fumigatus mat1-2. (A) Schematic representation of the targeted genomic region of recipient strain UI404 on Chr I. The A. nidulans ΔmatA allele on Chr III is also shown. Genetic organization of transformants created upon ectopic integration of A. fumigatus mat1-2 at the pyrG89 locus is shown in the box. Positions of restriction enzymes, predicted fragment size, and probes used to verify ectopic integration are indicated. (B) Southern blot analysis was performed to confirm A. fumigatus mat1-2 integration. Genomic DNA from A. nidulans WT and five transformants (T1 to T5) was digested with XbaI and probed with pyrG-specific probe 1. Transformants T1 and T5 were additionally verified with A. fumigatus mat1-2-specific probe 2. C, control.