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. 2008 Feb 1;7(6):1029–1040. doi: 10.1128/EC.00380-07

FIG. 5.

FIG. 5.

Sexual development in WT and ORF swap strains (UI421, UI422, and UI423). Cleistothecia were isolated, and the internal contents were examined at different time points after induction (see Materials and Methods). (A) WT at 6 days (6d) postinduction. Young ascus with immature ascospores (white arrow), and mature ascus with pigmented ascospores (black arrow). (B) WT at 8 days postinduction. Released mature ascospores (black arrow) and a few intact asci (white arrow). (C) WT at 12 days (12d) postinduction. Released mature ascospores (black arrow). (D) UI422 at 6 days postinduction. Young ascus (white arrow) and ascogenous hyphae (black arrow). (E) UI422 at 8 days postinduction. Immature ascus (white arrow) and mature ascus with ascospores (black arrow). (F) UI422 at 12 days postinduction. Released mature ascospores (white arrow) and young ascus (black arrow). (G) The phenotype of UI421 and UI423 up to 8 days postinduction is the same as UI422; however, at 12 days postinduction, development is completed and debris from undeveloped asci (white arrow) and ascospores (black arrow) are indicated. (H) UI422 at 24 days (24d) postinduction and released mature ascospores. Magnification bar, 20 μm.