FIG. 2.
Effect of AA on P. falciparum. (A) Inhibitory effect of sub-IC50s (5 and 10 μM) of AA on in vitro growth of parasite strains 3D7 and 7G8. Shown are average daily parasitemias determined from three replicates of synchronized culture with a 0.5% initial parasitemia. (B) Inhibition of intraerythrocytic growth of 3D7 P. falciparum. Shown is the gross morphology of Giemsa-stained parasites from control (DMSO) and AA-treated (at 20 or 50 μM) cultures. Note the growth retardation and vacuolated parasites, especially after 50 μM of AA treatment. (C) Proportions of each asexual erythrocytic stage at different times points in control (a) and AA-treated (b) cultures. Synchronized 12-h ring stage parasites at 0.5% parasitemia were treated with or without 20 μM of AA, and the proportion of each stage was obtained by counting 300 live parasites.