FIG. 6.
RT-PCR analysis of gene expression to validate results of the microarray analysis. (A) Semiquantitative RT-PCR was performed using cDNA synthesized from equal amounts of total RNA from parasites treated with 0, 5, 10, and 20 μM of AA for 12 h. Top, middle, and bottom panels show downregulated, upregulated, and invariant genes, respectively. For downregulated genes 22 cycles of PCR were performed, whereas 25 cycles were done for the rest of genes. (B) Real-time RT-PCR was performed, and the relative expression level was the 2−ΔΔCT value determined by comparing AA-treated parasites with untreated controls and using gene PF07_0047 as the internal control.